Yuki Yoshida

Yuki Yoshida

I am a JSPS PD fellow at The University of Tokyo. My interests include evolutionary genomics in metazoa and synthetic genomics in bacteria.

JSPS PD Fellow at Wakamoto lab, The University of Tokyo, JAPAN.

Effective chemical transfection for an anhydrobiotic insect cell line

Published in Cryobiology & Cryotechnology, 2025

The anhydrobiotic insect cell line Pv11, established from Polypedilum vanderplanki embryos, enables molecular studies of extreme desiccation tolerance. This study presents a chemical transfection method using FuGENE® HD as an alternative to electroporation. While requiring longer incubation, this approach markedly reduces cytotoxicity while maintaining robust gene delivery, providing a more practical method for genetic manipulation of Pv11 cells.

Recommended citation: Yoshida Y., Pratama G. A., Hotta M., and Kikawada T. (2025) Effective chemical transfection for an anhydrobiotic insect cell line. Cryobiol Cryotechnol, 71, 2:81-88.