High throughput genotype-phenotype linkage by Raman microscopy
Date:
Yuki Yoshida, Reiko Okura, Kenichiro Kamei, Yuichi Wakamoto. “High throughput genotype-phenotype linkage by Raman microscopy”.
Abstract
The establishment of comprehensive genetic resources, i.e., single gene knockout collection, has enabled comparative physiological and cell biological studies. Building on this foundation, we hypothesized that simultaneous profiling of such resources would provide further insights on genotype-phenotype linkage. We have previously observed that the Raman spectrum, a profile obtained by detecting cellular Raman scattering, reflects the cellular state in E. coli. Thus, we established a time-lapse Raman microscopy system using a Quartz microfluidic mother machine device and profiled various KEIO collection strains combined with FISH-based identification of each strain. In this presentation, we discuss our initial results obtained through this microscopic environment.
Keywords
Raman microscopy, E. coli, FISH, microfluidic device, Quartz